eef2k inhibitors Search Results


a484954  (Merck KGaA)
90
Merck KGaA a484954
SIRT1 activates eEF2K/eEF2-dependent autophagy in response to ER stress. ( A ) H9c2 cells were treated with 10 µM <t>A484954</t> for 4 h (eEF2K inhibitor) and the level of phospho-eEF2 (p-eEF2) was analyzed by Western blot (n = 7). The ratio of p-eEF2 vs. total eEF2 is presented. ( B ) H9c2 cells were left untreated or treated with 10 μg/mL tunicamycin (TN) for 48 h ± 10 µM A484954, and cell viability was determined by Fluorescein Diacetate (FDA) assay. Results presented in the bar graph are expressed as mean ± S.E.M. of percentages of dead cells (FDA negative cells, n = 11). ( C ) Autophagy was monitored by flow cytometry after Cyto-ID ® staining of H9c2 cells untreated or treated with 10 µg/mL tunicamycin (TN) for 24 h ± 10 µM A484954 (n = 11). ( D ) Cells were left untreated or treated with 10 μg/mL tunicamycin (TN) for 24 h ± 10 µM A484954 and the levels of LC3-II were analyzed by Western blot. Actin was used as a loading control. The relative mean expression level of LC3-II is presented in the bar graph. Error bars, S.E.M. (n = 5). ( E , F , I ) H9c2 cells were transfected with control, eEF2K or eEF2 siRNA, and eEF2K ( E ) or eEF2 ( I ) expression and eEF2 phosphorylation ( F , I ) were assessed after 24 h by Western blot. ( G , J ) H9c2 cells were transfected with control, eEF2K ( G ), or eEF2 ( J ) siRNA for 24 h, then treated for 48 h with tunicamycin (TN) and cell death (FDA negative cells) was measured by flow cytometry. Data in the bar graphs represent mean ± S.E.M. (n = 3). ( H , K ) H9c2 cells were transfected with control, eEF2K ( H ) or eEF2 ( K ) siRNA for 24 h, then treated for 24 h with tunicamycin (TN) and autophagy (MFI of Cyto-ID ® ) was measured by flow cytometry. Data in the bar graphs represent mean ± S.E.M. (n = 3). ( L ) phospho-eEF2 expression level was measured by Western blot in H9c2 cells after 4 h of 10 μg/mL tunicamycin (TN) ± 50µM EX527 (SIRT1 inhibitor) treatment. The ratio of p-eEF2 vs. total eEF2 is presented in the bar graph. Error bars, S.E.M. (n = 9). * P < 0.05, ** P < 0.01, *** P < 0.005 versus control. # P < 0.05, ### P < 0.005 versus TN.
A484954, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eef2k+inhibitors/eef2k+inhibitor++a+484954++7+amino+1+cyclopropyl+3+ethyl+2+4+dioxo+1+2+3+4+tetrahydropyrido+2+3+d++pyrimidine+6+carboxamide/pmc07072417-41-15-18
Average 90 stars, based on 1 article reviews
a484954 - by Bioz Stars, 2026-09
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eef2k inhibitors  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare eef2k inhibitors
SIRT1 activates eEF2K/eEF2-dependent autophagy in response to ER stress. ( A ) H9c2 cells were treated with 10 µM <t>A484954</t> for 4 h (eEF2K inhibitor) and the level of phospho-eEF2 (p-eEF2) was analyzed by Western blot (n = 7). The ratio of p-eEF2 vs. total eEF2 is presented. ( B ) H9c2 cells were left untreated or treated with 10 μg/mL tunicamycin (TN) for 48 h ± 10 µM A484954, and cell viability was determined by Fluorescein Diacetate (FDA) assay. Results presented in the bar graph are expressed as mean ± S.E.M. of percentages of dead cells (FDA negative cells, n = 11). ( C ) Autophagy was monitored by flow cytometry after Cyto-ID ® staining of H9c2 cells untreated or treated with 10 µg/mL tunicamycin (TN) for 24 h ± 10 µM A484954 (n = 11). ( D ) Cells were left untreated or treated with 10 μg/mL tunicamycin (TN) for 24 h ± 10 µM A484954 and the levels of LC3-II were analyzed by Western blot. Actin was used as a loading control. The relative mean expression level of LC3-II is presented in the bar graph. Error bars, S.E.M. (n = 5). ( E , F , I ) H9c2 cells were transfected with control, eEF2K or eEF2 siRNA, and eEF2K ( E ) or eEF2 ( I ) expression and eEF2 phosphorylation ( F , I ) were assessed after 24 h by Western blot. ( G , J ) H9c2 cells were transfected with control, eEF2K ( G ), or eEF2 ( J ) siRNA for 24 h, then treated for 48 h with tunicamycin (TN) and cell death (FDA negative cells) was measured by flow cytometry. Data in the bar graphs represent mean ± S.E.M. (n = 3). ( H , K ) H9c2 cells were transfected with control, eEF2K ( H ) or eEF2 ( K ) siRNA for 24 h, then treated for 24 h with tunicamycin (TN) and autophagy (MFI of Cyto-ID ® ) was measured by flow cytometry. Data in the bar graphs represent mean ± S.E.M. (n = 3). ( L ) phospho-eEF2 expression level was measured by Western blot in H9c2 cells after 4 h of 10 μg/mL tunicamycin (TN) ± 50µM EX527 (SIRT1 inhibitor) treatment. The ratio of p-eEF2 vs. total eEF2 is presented in the bar graph. Error bars, S.E.M. (n = 9). * P < 0.05, ** P < 0.01, *** P < 0.005 versus control. # P < 0.05, ### P < 0.005 versus TN.
Eef2k Inhibitors, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eef2k+inhibitors/eef2k+inhibitors/10__3233_slash_jad___140402-555-0-8
Average 90 stars, based on 1 article reviews
eef2k inhibitors - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


SIRT1 activates eEF2K/eEF2-dependent autophagy in response to ER stress. ( A ) H9c2 cells were treated with 10 µM A484954 for 4 h (eEF2K inhibitor) and the level of phospho-eEF2 (p-eEF2) was analyzed by Western blot (n = 7). The ratio of p-eEF2 vs. total eEF2 is presented. ( B ) H9c2 cells were left untreated or treated with 10 μg/mL tunicamycin (TN) for 48 h ± 10 µM A484954, and cell viability was determined by Fluorescein Diacetate (FDA) assay. Results presented in the bar graph are expressed as mean ± S.E.M. of percentages of dead cells (FDA negative cells, n = 11). ( C ) Autophagy was monitored by flow cytometry after Cyto-ID ® staining of H9c2 cells untreated or treated with 10 µg/mL tunicamycin (TN) for 24 h ± 10 µM A484954 (n = 11). ( D ) Cells were left untreated or treated with 10 μg/mL tunicamycin (TN) for 24 h ± 10 µM A484954 and the levels of LC3-II were analyzed by Western blot. Actin was used as a loading control. The relative mean expression level of LC3-II is presented in the bar graph. Error bars, S.E.M. (n = 5). ( E , F , I ) H9c2 cells were transfected with control, eEF2K or eEF2 siRNA, and eEF2K ( E ) or eEF2 ( I ) expression and eEF2 phosphorylation ( F , I ) were assessed after 24 h by Western blot. ( G , J ) H9c2 cells were transfected with control, eEF2K ( G ), or eEF2 ( J ) siRNA for 24 h, then treated for 48 h with tunicamycin (TN) and cell death (FDA negative cells) was measured by flow cytometry. Data in the bar graphs represent mean ± S.E.M. (n = 3). ( H , K ) H9c2 cells were transfected with control, eEF2K ( H ) or eEF2 ( K ) siRNA for 24 h, then treated for 24 h with tunicamycin (TN) and autophagy (MFI of Cyto-ID ® ) was measured by flow cytometry. Data in the bar graphs represent mean ± S.E.M. (n = 3). ( L ) phospho-eEF2 expression level was measured by Western blot in H9c2 cells after 4 h of 10 μg/mL tunicamycin (TN) ± 50µM EX527 (SIRT1 inhibitor) treatment. The ratio of p-eEF2 vs. total eEF2 is presented in the bar graph. Error bars, S.E.M. (n = 9). * P < 0.05, ** P < 0.01, *** P < 0.005 versus control. # P < 0.05, ### P < 0.005 versus TN.

Journal: Cells

Article Title: SIRT1 Protects the Heart from ER Stress-Induced Injury by Promoting eEF2K/eEF2-Dependent Autophagy

doi: 10.3390/cells9020426

Figure Lengend Snippet: SIRT1 activates eEF2K/eEF2-dependent autophagy in response to ER stress. ( A ) H9c2 cells were treated with 10 µM A484954 for 4 h (eEF2K inhibitor) and the level of phospho-eEF2 (p-eEF2) was analyzed by Western blot (n = 7). The ratio of p-eEF2 vs. total eEF2 is presented. ( B ) H9c2 cells were left untreated or treated with 10 μg/mL tunicamycin (TN) for 48 h ± 10 µM A484954, and cell viability was determined by Fluorescein Diacetate (FDA) assay. Results presented in the bar graph are expressed as mean ± S.E.M. of percentages of dead cells (FDA negative cells, n = 11). ( C ) Autophagy was monitored by flow cytometry after Cyto-ID ® staining of H9c2 cells untreated or treated with 10 µg/mL tunicamycin (TN) for 24 h ± 10 µM A484954 (n = 11). ( D ) Cells were left untreated or treated with 10 μg/mL tunicamycin (TN) for 24 h ± 10 µM A484954 and the levels of LC3-II were analyzed by Western blot. Actin was used as a loading control. The relative mean expression level of LC3-II is presented in the bar graph. Error bars, S.E.M. (n = 5). ( E , F , I ) H9c2 cells were transfected with control, eEF2K or eEF2 siRNA, and eEF2K ( E ) or eEF2 ( I ) expression and eEF2 phosphorylation ( F , I ) were assessed after 24 h by Western blot. ( G , J ) H9c2 cells were transfected with control, eEF2K ( G ), or eEF2 ( J ) siRNA for 24 h, then treated for 48 h with tunicamycin (TN) and cell death (FDA negative cells) was measured by flow cytometry. Data in the bar graphs represent mean ± S.E.M. (n = 3). ( H , K ) H9c2 cells were transfected with control, eEF2K ( H ) or eEF2 ( K ) siRNA for 24 h, then treated for 24 h with tunicamycin (TN) and autophagy (MFI of Cyto-ID ® ) was measured by flow cytometry. Data in the bar graphs represent mean ± S.E.M. (n = 3). ( L ) phospho-eEF2 expression level was measured by Western blot in H9c2 cells after 4 h of 10 μg/mL tunicamycin (TN) ± 50µM EX527 (SIRT1 inhibitor) treatment. The ratio of p-eEF2 vs. total eEF2 is presented in the bar graph. Error bars, S.E.M. (n = 9). * P < 0.05, ** P < 0.01, *** P < 0.005 versus control. # P < 0.05, ### P < 0.005 versus TN.

Article Snippet: EX527 was from Tocris (49843-98-3), STAC-3, and Endothall were from Santa Cruz (sc-222315 and sc-201325), A484954 was from Merck Chemicals (324516).

Techniques: Western Blot, Flow Cytometry, Staining, Control, Expressing, Transfection, Phospho-proteomics